Acridine Orange (AO) is a cell-permeant dye that stains nucleic
acids (RNA and DNA), providing a means of generically labeling
nucleated cells in samples. When bound to DNA, AO can be excited
with a 488nm laser and will fluorescently emit at a 525nm
maximum, making it well-suited for detection with the PMT1
(525/45nm) channel of the Moxi GO
II. The cells can be counterstained with Propidium Iodide
(PI) to simultaneously determine the sample viability using the
well-established membrane permeability metric.
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